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Human B lymphoblastoid cell line(HMy2.C1R)
Human B lymphoblastoid cell line(HMy2.C1R) 
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Cat.No.： TCH-C206 Size： 1x10^6

The HMy2.CIR cell line is a variant derived from the fast-growing mutant Hmy.2B of the ARH-77 cell line, obtained through γ-ray irradiation and selection for cells lacking expression of HLA class I antigens. The HMy2.CIR cells do not express products of the HLA-A or HLA-B loci but exhibit low-level expression of HLA-Cw4. This cell line is suitable as a host for transfection of type I major histocompatibility complex (MHC) antigen genes. It has been reported that the ARH-77 cell line is positive for Epstein-Barr nuclear antigen (EBNA+) and Epstein-Barr virus capsid antigen (EBVCA+). Since the HMy2.CIR cell line originates from the fast-growing mutant Hmy.2B of the ARH-77 cell line, it is presumed that HMy2.CIR is also EBNA+.


Cell Name: Human B lymphoblastoid cell line

Cell Synonyms: HMY2.CIR; Hmy.2 CIR; HMy2.CIR; C1R

Catalogue No.: TCH-C206
Species: Human
Tissue Origin: B Lymphoblast
Disease Characteristics: —
Morphology: Lymphoblast-like
Growth Characteristics: Suspension
Culture Medium: IMDM + 10% FBS + 1% P/S
Matching Medium Catalog Number: TCH-G206

Passage Ratio: 3×105-1×106 cells/mL, with medium renewal every 2-3 days
Doubling Time: 24-36 h
Culture Conditions: Atmosphere: 95% air + 5% CO₂, Temperature: 37°C

FreezingConditions:   60% basal medium + 30% FBS + 10% DMSO, stored in liquid nitrogen

Recommended HyCyte® One-Step Cryopreservation Medium (ready-to-use, serum-free, no programmed cooling required), Product No.: GUCP-R201
Quality Control: Negative for bacteria, fungi, and mycoplasma detection

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